February 07, 2017 at 12:25 PM

The Dickman group has developed analytical methods that enable the rapid purification of dsRNA from associated impurities from bacterial cells in conjunction with downstream analyses. We have optimised base-specific cleavage of dsRNA by RNase A and developed a novel method utilising RNase T1 for RNase mass mapping approaches to further characterise the dsRNA using liquid chromatography interfaced with mass spectrometry.

Highlights
• rapid purification of dsRNA in a single step protocol.
• high throughput purification and analysis of a wide range of dsRNAs.
• developed IP RP HPLC for the rapid, high resolution analysis of the dsRNA.
• developed a novel method utilising RNase T1 for RNase mass mapping of dsRNA.

This work was performed in collaboration with Syngenta.

Full paper is available here.


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