The Dickman group has developed analytical methods that enable the rapid purification of dsRNA from associated impurities from bacterial cells in conjunction with downstream analyses. We have optimised base-specific cleavage of dsRNA by RNase A and developed a novel method utilising RNase T1 for RNase mass mapping approaches to further characterise the dsRNA using liquid chromatography interfaced with mass spectrometry.
• rapid purification of dsRNA in a single step protocol.
• high throughput purification and analysis of a wide range of dsRNAs.
• developed IP RP HPLC for the rapid, high resolution analysis of the dsRNA.
• developed a novel method utilising RNase T1 for RNase mass mapping of dsRNA.
This work was performed in collaboration with Syngenta.
Full paper is available here.