Professor Mark Dickman's group have investigated a range of different pore-sizes and phases of superficially porous particles for the analysis of a diverse range of nucleic acids including oligonucleotides, oligoribonucleotides, phosphorothioate oligonucleotides and high molecular weight dsDNA and RNA. This work demonstrated that the pore size of the superficially porous silica particles significantly effects resolution. Furthermore, they utilised 150 Å pore size solid-core particles to separate typical impurities generated in the synthesis of therapeutic oligonucleotides which are utilised as drugs to treat a wide range of diseases.
This work was performed in conjunction with industrial partners GSK and Thermo Fisher Scientific.
The full paper can be read here.